› 1em1

X-ray diffraction
2.13Å resolution

X-RAY CRYSTAL STRUCTURE FOR HUMAN MANGANESE SUPEROXIDE DISMUTASE, Q143A

Released:
DOI: 10.2210/pdb1em1/pdb
PDB entry 1em1 coloured by chain, front view.
PDB entry 1em1 coloured by chain, side view.
PDB entry 1em1 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Multiple replacements of glutamine 143 in human manganese superoxide dismutase: effects on structure, stability, and catalysis.
Lévêque VJ, Stroupe ME, Lepock JR, Cabelli DE, Tainer JA, Nick HS, Silverman DN
Biochemistry 39 7131-7 (2000)
PMID: 10852710

Function and Biology Details

Reaction catalysed: 
2 superoxide + 2 H(+) = O(2) + H(2)O(2)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-137485 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Superoxide dismutase [Mn], mitochondrial Chains: A, B
Molecule details ›
Chains: A, B
Length: 198 amino acids
Theoretical weight: 22.18 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P04179 (Residues: 25-222; Coverage: 89%)
Gene name: SOD2
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand SO4 2 x SO4
Ligand MN 2 x MN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL7-1
Spacegroup: P6122
Unit cell:
a: 79.643Å b: 79.643Å c: 241.642Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.232 0.232 0.264
Expression system: Escherichia coli

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Citations

5 review citations

Carbonic anhydrase as a model for biophysical and physical-organic studies of proteins and protein-ligand binding.
Krishnamurthy et al. (2008)
4 more

2 mentions without citation

Superoxide dismutases and superoxide reductases.
Sheng et al. (2014)
1 more