› 1em6

X-ray diffraction
2.2Å resolution

HUMAN LIVER GLYCOGEN PHOSPHORYLASE A COMPLEXED WITH GLCNAC AND CP-526,423

Released:
DOI: 10.2210/pdb1em6/pdb
PDB entry 1em6 coloured by chain, front view.
PDB entry 1em6 coloured by chain, side view.
PDB entry 1em6 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Human liver glycogen phosphorylase inhibitors bind at a new allosteric site.
Rath VL, Ammirati M, Danley DE, Ekstrom JL, Gibbs EM, Hynes TR, Mathiowetz AM, McPherson RK, Olson TV, Treadway JL, Hoover DJ
Chem Biol 7 677-82 (2000)
PMID: 10980448

Function and Biology Details

Reaction catalysed: 
((1->4)-alpha-D-glucosyl)(n) + phosphate = ((1->4)-alpha-D-glucosyl)(n-1) + alpha-D-glucose 1-phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139193 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glycogen phosphorylase, liver form Chains: A, B
Molecule details ›
Chains: A, B
Length: 847 amino acids
Theoretical weight: 97.28 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P06737 (Residues: 1-847; Coverage: 100%)
Gene name: PYGL
Sequence domains: Carbohydrate phosphorylase
Structure domains: Glycogen Phosphorylase B;

Ligands and Environments


Cofactor: Ligand PLP 2 x PLP
3 bound ligands:
Ligand NBG 2 x NBG
Ligand CP4 1 x CP4
Ligand MPD 1 x MPD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X12C
Spacegroup: P31
Unit cell:
a: 123.314Å b: 123.314Å c: 122.319Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.237 0.233 0.264
Expression system: Spodoptera frugiperda

Quick links

Citations

15 review citations

Implications of protein flexibility for drug discovery.
Teague SJ. (2003)
14 more

1 mention without citation

In silico and in vitro studies of lupeol and iso-orientin as potential antidiabetic agents in a rat model.
Malik et al. (2019)