› 1esk

Solution NMR

SOLUTION STRUCTURE OF NCP7 FROM HIV-1

Released:
DOI: 10.2210/pdb1esk/pdb
PDB entry 1esk coloured by chain, ensemble of 9 models, front view.
PDB entry 1esk coloured by chain, ensemble of 9 models, side view.
PDB entry 1esk coloured by chain, ensemble of 9 models, top view.
Source organism: HIV-1 M:B_HXB2R
Primary publication:
Solution Structure of (12-53)NCp7 of HIV-1
Morellet N, Demene H, Teilleux V, Huynh-Dinh T, de Rocquigny H, Fournie-Zaluski M-C, Roques BP
To be published

Function and Biology Details

Reactions catalysed: 
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
3'-end directed exonucleolytic cleavage of viral RNA-DNA hybrid
Endohydrolysis of RNA in RNA/DNA hybrids. Three different cleavage modes: 1. sequence-specific internal cleavage of RNA. Human immunodeficiency virus type 1 and Moloney murine leukemia virus enzymes prefer to cleave the RNA strand one nucleotide away from the RNA-DNA junction. 2. RNA 5'-end directed cleavage 13-19 nucleotides from the RNA end. 3. DNA 3'-end directed cleavage 15-20 nucleotides away from the primer terminus.
Specific for a P1 residue that is hydrophobic, and P1' variable, but often Pro.
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-138004 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Nucleocapsid protein p7 Chain: A
Molecule details ›
Chain: A
Length: 42 amino acids
Theoretical weight: 4.84 KDa
Source organism: HIV-1 M:B_HXB2R
Expression system: Not provided
UniProt:
  • Canonical: P04585 (Residues: 389-430; Coverage: 3%)
Gene name: gag-pol
Sequence domains: Zinc knuckle
Structure domains: Zinc finger, CCHC-type

Ligands and Environments

1 bound ligand:
Ligand ZN 2 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: simulated annealing
Expression system: Not provided

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