› 1eym

X-ray diffraction
2Å resolution

FK506 BINDING PROTEIN MUTANT, HOMODIMERIC COMPLEX

Released:
DOI: 10.2210/pdb1eym/pdb
PDB entry 1eym coloured by chain, front view.
PDB entry 1eym coloured by chain, side view.
PDB entry 1eym coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
A ligand-reversible dimerization system for controlling protein-protein interactions.
Rollins CT, Rivera VM, Woolfson DN, Keenan T, Hatada M, Adams SE, Andrade LJ, Yaeger D, van Schravendijk MR, Holt DA, Gilman M, Clackson T
Proc Natl Acad Sci U S A 97 7096-101 (2000)
PMID: 10852943

Function and Biology Details

Reaction catalysed: 
Peptidylproline (omega=180) = peptidylproline (omega=0)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-158814 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Peptidyl-prolyl cis-trans isomerase FKBP1A Chains: A, B
Molecule details ›
Chains: A, B
Length: 107 amino acids
Theoretical weight: 11.82 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P62942 (Residues: 2-108; Coverage: 99%)
Gene names: FKBP1, FKBP12, FKBP1A
Sequence domains: FKBP-type peptidyl-prolyl cis-trans isomerase
Structure domains: Chitinase A; domain 3

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P43212
Unit cell:
a: 81.68Å b: 81.68Å c: 93.94Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.23 0.23 0.29
Expression system: Escherichia coli

Quick links

Citations

28 review citations

Protein translocation as a tool: The current rapamycin story.
Putyrski et al. (2012)
27 more

2 mentions without citation

A ligand-reversible dimerization system for controlling protein-protein interactions.
Rollins et al. (2000)
1 more