› 1f1j

X-ray diffraction
2.35Å resolution

CRYSTAL STRUCTURE OF CASPASE-7 IN COMPLEX WITH ACETYL-ASP-GLU-VAL-ASP-CHO

Released:
DOI: 10.2210/pdb1f1j/pdb
PDB entry 1f1j coloured by chain, front view.
PDB entry 1f1j coloured by chain, side view.
PDB entry 1f1j coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The structures of caspases-1, -3, -7 and -8 reveal the basis for substrate and inhibitor selectivity.
Wei Y, Fox T, Chambers SP, Sintchak J, Coll JT, Golec JM, Swenson L, Wilson KP, Charifson PS
Chem Biol 7 423-32 (2000)
PMID: 10873833

Function and Biology Details

Reaction catalysed: 
Strict requirement for an Asp residue at position P1 and has a preferred cleavage sequence of Asp-Glu-Val-Asp-|-
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
hetero dimer (preferred)
hetero octamer
hetero tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-157231 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Caspase-7 Chains: A, B
Molecule details ›
Chains: A, B
Length: 305 amino acids
Theoretical weight: 34.55 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P55210 (Residues: 1-303; Coverage: 100%)
Gene names: CASP7, MCH3
Sequence domains: Caspase domain
Structure domains: Rossmann fold
ACE-ASP-GLU-VAL-ASP-CHO Chains: C, D
Molecule details ›
Chains: C, D
Length: 5 amino acids
Theoretical weight: 488 Da
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand SO4 2 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: P3221
Unit cell:
a: 88.18Å b: 88.18Å c: 186.23Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.185 0.185 0.263
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

23 review citations

Molecular mechanisms of caspase regulation during apoptosis.
Riedl et al. (2004)
22 more

44 mentions without citation

The protein structures that shape caspase activity, specificity, activation and inhibition.
Fuentes-Prior et al. (2004)
43 more