› 1f5n

X-ray diffraction
1.7Å resolution

HUMAN GUANYLATE BINDING PROTEIN-1 IN COMPLEX WITH THE GTP ANALOGUE, GMPPNP.

Released:
DOI: 10.2210/pdb1f5n/pdb
PDB entry 1f5n coloured by chain, front view.
PDB entry 1f5n coloured by chain, side view.
PDB entry 1f5n coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Triphosphate structure of guanylate-binding protein 1 and implications for nucleotide binding and GTPase mechanism.
Prakash B, Renault L, Praefcke GJ, Herrmann C, Wittinghofer A
EMBO J 19 4555-64 (2000)
PMID: 10970849

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152319 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Guanylate-binding protein 1 Chain: A
Molecule details ›
Chain: A
Length: 592 amino acids
Theoretical weight: 68 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P32455 (Residues: 1-592; Coverage: 100%)
Gene name: GBP1
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand MG 1 x MG
Ligand GNP 1 x GNP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID2
Spacegroup: C2
Unit cell:
a: 161.228Å b: 42.652Å c: 91.544Å
α: 90° β: 100.19° γ: 90°
R-values:
R R work R free
0.224 0.226 0.255
Expression system: Escherichia coli

Quick links

Citations

20 review citations

The dynamin superfamily: universal membrane tubulation and fission molecules?
Praefcke et al. (2004)
19 more

30 mentions without citation

Interferon-Inducible GTPases in Host Resistance, Inflammation and Disease.
Pilla-Moffett et al. (2016)
29 more