› 1f6l

X-ray diffraction
2.8Å resolution

VARIABLE LIGHT CHAIN DIMER OF ANTI-FERRITIN ANTIBODY

Released:
DOI: 10.2210/pdb1f6l/pdb
PDB entry 1f6l coloured by chain, front view.
PDB entry 1f6l coloured by chain, side view.
PDB entry 1f6l coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Antiferritin VL homodimer binds human spleen ferritin with high specificity.
Nymalm Y, Kravchuk Z, Salminen T, Chumanevich AA, Dubnovitsky AP, Kankare J, Pentikäinen O, Lehtonen J, Arosio P, Martsev S, Johnson MS
J Struct Biol 138 171-86 (2002)
PMID: 12217656

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-134447 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Immunoglobulin kappa variable 1-39 Chain: L
Molecule details ›
Chain: L
Length: 114 amino acids
Theoretical weight: 12.71 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P01597 (Residues: 23-117; Coverage: 100%)
Gene name: IGKV1-39
Sequence domains: Immunoglobulin V-set domain
Structure domains: Immunoglobulins

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P6122
Unit cell:
a: 52.214Å b: 52.214Å c: 157.75Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.191 0.191 0.257
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Development trends for generation of single-chain antibody fragments.
Farajnia et al. (2014)
5 other articles

1 mention without citation

Thermodynamic stability and flexibility characteristics of antibody fragment complexes.
Li et al. (2014)