› 1f6o

X-ray diffraction
2.4Å resolution

CRYSTAL STRUCTURE OF THE HUMAN AAG DNA REPAIR GLYCOSYLASE COMPLEXED WITH DNA

Released:
DOI: 10.2210/pdb1f6o/pdb
PDB entry 1f6o coloured by chain, front view.
PDB entry 1f6o coloured by chain, side view.
PDB entry 1f6o coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Molecular basis for discriminating between normal and damaged bases by the human alkyladenine glycosylase, AAG.
Lau AY, Wyatt MD, Glassner BJ, Samson LD, Ellenberger T
Proc Natl Acad Sci U S A 97 13573-8 (2000)
PMID: 11106395

Function and Biology Details

Reaction catalysed: 
Hydrolysis of alkylated DNA, releasing 3-methyladenine, 3-methylguanine, 7-methylguanine and 7-methyladenine
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-113798 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
DNA-3-methyladenine glycosylase Chain: A
Molecule details ›
Chain: A
Length: 219 amino acids
Theoretical weight: 24.33 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P29372 (Residues: 80-298; Coverage: 74%)
Gene names: AAG, ANPG, MID1, MPG
Sequence domains: Methylpurine-DNA glycosylase (MPG)
Structure domains: Methylpurine-DNA glycosylase (MPG)
DNA (5'-D(*GP*AP*CP*AP*TP*GP*(YRR)P*TP*TP*GP*CP*CP*T)-3') Chain: D
Molecule details ›
Chain: D
Length: 13 nucleotides
Theoretical weight: 3.83 KDa
Source organism: Homo sapiens
Expression system: Not provided
DNA (5'-D(*GP*GP*CP*AP*AP*TP*CP*AP*TP*GP*TP*CP*A)-3') Chain: E
Molecule details ›
Chain: E
Length: 13 nucleotides
Theoretical weight: 3.98 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand NA 1 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X12C
Spacegroup: P212121
Unit cell:
a: 43.75Å b: 57.1Å c: 128.45Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.219 0.219 0.282
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

31 review citations

Genome editing with CRISPR-Cas nucleases, base editors, transposases and prime editors.
Anzalone et al. (2020)
30 more

5 mentions without citation

Detection of damaged DNA bases by DNA glycosylase enzymes.
Friedman et al. (2010)
4 more