› 1f93

X-ray diffraction
2.6Å resolution

CRYSTAL STRUCTURE OF A COMPLEX BETWEEN THE DIMERIZATION DOMAIN OF HNF-1 ALPHA AND THE COACTIVATOR DCOH

Released:
DOI: 10.2210/pdb1f93/pdb
PDB entry 1f93 coloured by chain, front view.
PDB entry 1f93 coloured by chain, side view.
PDB entry 1f93 coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis of dimerization, coactivator recognition and MODY3 mutations in HNF-1alpha.
Rose RB, Bayle JH, Endrizzi JA, Cronk JD, Crabtree GR, Alber T
Nat Struct Biol 7 744-8 (2000)
PMID: 10966642

Function and Biology Details

Reaction catalysed: 
4a-hydroxytetrahydrobiopterin = 6,7-dihydrobiopterin + H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149605 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Pterin-4-alpha-carbinolamine dehydratase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 104 amino acids
Theoretical weight: 12.16 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: P61459 (Residues: 1-104; Coverage: 100%)
Gene names: Dcoh, Pcbd, Pcbd1
Sequence domains: Pterin 4 alpha carbinolamine dehydratase
Structure domains: Transcriptional coactivator/pterin dehydratase
Hepatocyte nuclear factor 1-alpha Chains: E, F, G, H
Molecule details ›
Chains: E, F, G, H
Length: 32 amino acids
Theoretical weight: 3.39 KDa
Source organism: Mus musculus
Expression system: Not provided
UniProt:
  • Canonical: P22361 (Residues: 1-32; Coverage: 5%)
Gene names: Hnf-1, Hnf-1a, Hnf1a, Tcf1

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 12 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL1-5
Spacegroup: P21
Unit cell:
a: 49.48Å b: 82.75Å c: 70.64Å
α: 90° β: 97.83° γ: 90°
R-values:
R R work R free
0.26 0.257 0.299
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

3 review citations

Eukaryotic transcription factors.
Warren AJ. (2002)
2 more

4 mentions without citation

Mutations in PCBD1 cause hypomagnesemia and renal magnesium wasting.
Ferrè et al. (2014)
3 more