› 1fao

X-ray diffraction
1.8Å resolution

STRUCTURE OF THE PLECKSTRIN HOMOLOGY DOMAIN FROM DAPP1/PHISH IN COMPLEX WITH INOSITOL 1,3,4,5-TETRAKISPHOSPHATE

Released:
DOI: 10.2210/pdb1fao/pdb
PDB entry 1fao coloured by chain, front view.
PDB entry 1fao coloured by chain, side view.
PDB entry 1fao coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis for discrimination of 3-phosphoinositides by pleckstrin homology domains.
Ferguson KM, Kavran JM, Sankaran VG, Fournier E, Isakoff SJ, Skolnik EY, Lemmon MA
Mol Cell 6 373-84 (2000)
PMID: 10983984

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-193982 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Dual adapter for phosphotyrosine and 3-phosphotyrosine and 3-phosphoinositide Chain: A
Molecule details ›
Chain: A
Length: 126 amino acids
Theoretical weight: 14.81 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9UN19 (Residues: 148-273; Coverage: 45%)
Gene names: BAM32, DAPP1, HSPC066
Sequence domains: PH domain
Structure domains: Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)

Ligands and Environments

1 bound ligand:
Ligand 4IP 1 x 4IP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: P212121
Unit cell:
a: 40.674Å b: 46.273Å c: 64.743Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.219 0.219 0.232
Expression system: Escherichia coli

Quick links

Citations

43 review citations

The activation of Akt/PKB signaling pathway and cell survival.
Song et al. (2005)
42 more

36 mentions without citation

Translation of the phosphoinositide code by PI effectors.
Kutateladze TG. (2010)
35 more