› 1fdt

X-ray diffraction
2.2Å resolution

HUMAN 17-BETA-HYDROXYSTEROID-DEHYDROGENASE TYPE 1 COMPLEXED WITH ESTRADIOL AND NADP+

Released:
DOI: 10.2210/pdb1fdt/pdb
PDB entry 1fdt coloured by chain, front view.
PDB entry 1fdt coloured by chain, side view.
PDB entry 1fdt coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The structure of a complex of human 17beta-hydroxysteroid dehydrogenase with estradiol and NADP+ identifies two principal targets for the design of inhibitors.
Breton R, Housset D, Mazza C, Fontecilla-Camps JC
Structure 4 905-15 (1996)
PMID: 8805577

Function and Biology Details

Reactions catalysed: 
Testosterone + NAD(P)(+) = androst-4-ene-3,17-dione + NAD(P)H
17-beta-estradiol + NAD(P)(+) = estrone + NAD(P)H
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146840 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
17-beta-hydroxysteroid dehydrogenase type 1 Chain: A
Molecule details ›
Chain: A
Length: 327 amino acids
Theoretical weight: 34.97 KDa
Source organism: Homo sapiens
Expression system: unidentified baculovirus
UniProt:
  • Canonical: P14061 (Residues: 2-328; Coverage: 100%)
Gene names: E17KSR, EDH17B1, EDH17B2, EDHB17, HSD17B1, SDR28C1
Sequence domains: short chain dehydrogenase
Structure domains: NAD(P)-binding Rossmann-like Domain

Ligands and Environments


Cofactor: Ligand NAP 1 x NAP
2 bound ligands:
Ligand SO4 1 x SO4
Ligand EST 1 x EST
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH2R
Spacegroup: C2
Unit cell:
a: 122.77Å b: 43.79Å c: 60.53Å
α: 90° β: 99.47° γ: 90°
R-values:
R R work R free
0.193 0.193 0.243
Expression system: unidentified baculovirus

Quick links

Citations

13 review citations

Molecular endocrinology of hydroxysteroid dehydrogenases.
Penning TM. (1997)
12 more

16 mentions without citation

The structural biology of oestrogen metabolism.
Thomas et al. (2013)
15 more