› 1fdv

X-ray diffraction
3.1Å resolution

HUMAN 17-BETA-HYDROXYSTEROID-DEHYDROGENASE TYPE 1 MUTANT H221L COMPLEXED WITH NAD+

Released:
DOI: 10.2210/pdb1fdv/pdb
PDB entry 1fdv coloured by chain, front view.
PDB entry 1fdv coloured by chain, side view.
PDB entry 1fdv coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Unusual charge stabilization of NADP+ in 17beta-hydroxysteroid dehydrogenase.
Mazza C, Breton R, Housset D, Fontecilla-Camps JC
J Biol Chem 273 8145-52 (1998)
PMID: 9525918

Function and Biology Details

Reactions catalysed: 
Testosterone + NAD(P)(+) = androst-4-ene-3,17-dione + NAD(P)H
17-beta-estradiol + NAD(P)(+) = estrone + NAD(P)H
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146840 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
17-beta-hydroxysteroid dehydrogenase type 1 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 327 amino acids
Theoretical weight: 34.95 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P14061 (Residues: 2-328; Coverage: 100%)
Gene names: E17KSR, EDH17B1, EDH17B2, EDHB17, HSD17B1, SDR28C1
Sequence domains: short chain dehydrogenase
Structure domains: NAD(P)-binding Rossmann-like Domain

Ligands and Environments


Cofactor: Ligand NAD 4 x NAD
1 bound ligand:
Ligand SO4 4 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM02
Spacegroup: P21
Unit cell:
a: 115.12Å b: 79.11Å c: 120.47Å
α: 90° β: 91.93° γ: 90°
R-values:
R R work R free
0.219 0.219 0.295
Expression system: Spodoptera frugiperda

Quick links

Citations

4 review citations

Integrated view on 17beta-hydroxysteroid dehydrogenases.
Moeller et al. (2009)
3 more

7 mentions without citation

The structural biology of oestrogen metabolism.
Thomas et al. (2013)
6 more