› 1fgu

X-ray diffraction
2.5Å resolution

SSDNA-BINDING DOMAIN OF THE LARGE SUBUNIT OF REPLICATION PROTEIN A

Released:
DOI: 10.2210/pdb1fgu/pdb
PDB entry 1fgu coloured by chain, front view.
PDB entry 1fgu coloured by chain, side view.
PDB entry 1fgu coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure of the major single-stranded DNA-binding domain of replication protein A suggests a dynamic mechanism for DNA binding.
Bochkareva E, Belegu V, Korolev S, Bochkarev A
EMBO J 20 612-8 (2001)
PMID: 11157767

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151052 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Replication protein A 70 kDa DNA-binding subunit, N-terminally processed Chains: A, B
Molecule details ›
Chains: A, B
Length: 252 amino acids
Theoretical weight: 28.11 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P27694 (Residues: 181-432; Coverage: 41%)
Gene names: REPA1, RPA1, RPA70
Sequence domains:
Structure domains: Nucleic acid-binding proteins

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P212121
Unit cell:
a: 63.5Å b: 84.86Å c: 119.11Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.215 0.215 0.281
Expression system: Escherichia coli

Quick links

Citations

24 review citations

A dynamic model for replication protein A (RPA) function in DNA processing pathways.
Fanning et al. (2006)
23 more

22 mentions without citation

Nucleic acid recognition by OB-fold proteins.
Theobald et al. (2003)
21 more