› 1fzd

X-ray diffraction
2.1Å resolution

STRUCTURE OF RECOMBINANT ALPHAEC DOMAIN FROM HUMAN FIBRINOGEN-420

Released:
DOI: 10.2210/pdb1fzd/pdb
PDB entry 1fzd coloured by chain, front view.
PDB entry 1fzd coloured by chain, side view.
PDB entry 1fzd coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure of a recombinant alphaEC domain from human fibrinogen-420.
Spraggon G, Applegate D, Everse SJ, Zhang JZ, Veerapandian L, Redman C, Doolittle RF, Grieninger G
Proc Natl Acad Sci U S A 95 9099-104 (1998)
PMID: 9689040

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136028 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (4 distinct):
Fibrinogen alpha chain Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 201 amino acids
Theoretical weight: 22.84 KDa
Source organism: Homo sapiens
Expression system: Komagataella pastoris
UniProt:
  • Canonical: P02671 (Residues: 666-866; Coverage: 24%)
Gene name: FGA
Sequence domains: Fibrinogen beta and gamma chains, C-terminal globular domain
Structure domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, MAN
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, MAN, NDG
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
2 bound ligands:
Ligand CA 8 x CA
Ligand NAG 4 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X12C
Spacegroup: P1
Unit cell:
a: 71.25Å b: 105.18Å c: 71.14Å
α: 104.6° β: 108.95° γ: 71.47°
R-values:
R R work R free
0.199 0.195 0.255
Expression system: Komagataella pastoris

Quick links

Citations

9 review citations

Heterologous protein expression in the methylotrophic yeast Pichia pastoris.
Cereghino et al. (2000)
8 more

4 mentions without citation

Crystal structure of a recombinant alphaEC domain from human fibrinogen-420.
Spraggon et al. (1998)
3 more