› 1g17

X-ray diffraction
2Å resolution

CRYSTAL STRUCTURE OF SEC4-GUANOSINE-5'-(BETA,GAMMA)-IMIDOTRIPHOSPHATE

Released:
DOI: 10.2210/pdb1g17/pdb
PDB entry 1g17 coloured by chain, front view.
PDB entry 1g17 coloured by chain, side view.
PDB entry 1g17 coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Crystal structures of a Rab protein in its inactive and active conformations.
Stroupe C, Brunger AT
J Mol Biol 304 585-98 (2000)
PMID: 11099382

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139624 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ras-related protein SEC4 Chains: A, B
Molecule details ›
Chains: A, B
Length: 170 amino acids
Theoretical weight: 19.16 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P07560 (Residues: 18-187; Coverage: 79%)
Gene names: SEC4, SRO6, YFL005W
Sequence domains: Ras family
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

2 bound ligands:
Ligand MG 2 x MG
Ligand GNP 2 x GNP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.1
Spacegroup: P65
Unit cell:
a: 84.504Å b: 84.504Å c: 86.469Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.264 0.264 0.292
Expression system: Escherichia coli

Quick links

Citations

17 review citations

The Rab GTPase family.
Stenmark et al. (2001)
16 more

13 mentions without citation

Structural mechanisms for regulation of membrane traffic by rab GTPases.
Lee et al. (2009)
12 more