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X-ray diffraction
2.2Å resolution

CRYSTAL STRUCTURE OF PROTEIN TYROSINE PHOSPHATASE 1B COMPLEXED WITH A MONO-PHOSPHORYLATED PEPTIDE (ETDY(PTR)RKGGKGLL) FROM THE INSULIN RECEPTOR KINASE

Released:
DOI: 10.2210/pdb1g1g/pdb
PDB entry 1g1g coloured by chain, front view.
PDB entry 1g1g coloured by chain, side view.
PDB entry 1g1g coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Molecular basis for the dephosphorylation of the activation segment of the insulin receptor by protein tyrosine phosphatase 1B.
Salmeen A, Andersen JN, Myers MP, Tonks NK, Barford D
Mol Cell 6 1401-12 (2000)
PMID: 11163213

Function and Biology Details

Reaction catalysed: 
Protein tyrosine phosphate + H(2)O = protein tyrosine + phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-148264 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Tyrosine-protein phosphatase non-receptor type 1 Chain: A
Molecule details ›
Chain: A
Length: 298 amino acids
Theoretical weight: 34.69 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P18031 (Residues: 1-298; Coverage: 69%)
Gene names: PTP1B, PTPN1
Sequence domains: Protein-tyrosine phosphatase
Structure domains: Protein tyrosine phosphatase superfamily
MONO-PHOSPHORYLATED PEPTIDE FROM THE INSULIN RECEPTOR KINASE Chain: B
Molecule details ›
Chain: B
Length: 13 amino acids
Theoretical weight: 1.58 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

No bound ligands
1 modified residue:
Ligand PTR 1 x PTR

Experiments and Validation Details

Entry percentile scores
X-ray source: SRS BEAMLINE PX9.6
Spacegroup: P21
Unit cell:
a: 44.492Å b: 89.281Å c: 52.922Å
α: 90° β: 99.73° γ: 90°
R-values:
R R work R free
0.212 0.212 0.228
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

61 review citations

Protein tyrosine phosphatases in the human genome.
Alonso et al. (2004)
60 more

4 mentions without citation

Insights into the reaction of protein-tyrosine phosphatase 1B: crystal structures for transition state analogs of both catalytic steps.
Brandão et al. (2010)
3 more