› 1g1s

X-ray diffraction
1.9Å resolution

P-SELECTIN LECTIN/EGF DOMAINS COMPLEXED WITH PSGL-1 PEPTIDE

Released:
DOI: 10.2210/pdb1g1s/pdb
PDB entry 1g1s coloured by chain, front view.
PDB entry 1g1s coloured by chain, side view.
PDB entry 1g1s coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Insights into the molecular basis of leukocyte tethering and rolling revealed by structures of P- and E-selectin bound to SLe(X) and PSGL-1.
Somers WS, Tang J, Shaw GD, Camphausen RT
Cell 103 467-79 (2000)
PMID: 11081633

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-147621 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (3 distinct):
P-selectin Chains: A, B
Molecule details ›
Chains: A, B
Length: 162 amino acids
Theoretical weight: 19.13 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
UniProt:
  • Canonical: P16109 (Residues: 42-199; Coverage: 20%)
Gene names: GMRP, GRMP, SELP
Sequence domains: Lectin C-type domain
Structure domains:
P-selectin glycoprotein ligand 1 Chains: C, D
Molecule details ›
Chains: C, D
Length: 28 amino acids
Theoretical weight: 3.67 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
UniProt:
  • Canonical: Q14242 (Residues: 42-60; Coverage: 5%)
Gene name: SELPLG

Ligands and Environments

Carbohydrate polymer : NEW Components: NGA, NAG, GAL, SIA, FUC
Carbohydrate polymer
3 bound ligands:
Ligand SR 2 x SR
Ligand MRD 7 x MRD
Ligand NA 2 x NA
1 modified residue:
Ligand TYS 6 x TYS

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4A
Spacegroup: I222
Unit cell:
a: 63.445Å b: 96.756Å c: 187.289Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.204 0.204 0.235
Expression system: Cricetulus griseus

Quick links

Citations

91 review citations

Neutrophils, from marrow to microbes.
Borregaard N. (2010)
90 more

43 mentions without citation

Rolling cell adhesion.
McEver et al. (2010)
42 more