› 1gcz

X-ray diffraction
1.9Å resolution

MACROPHAGE MIGRATION INHIBITORY FACTOR (MIF) COMPLEXED WITH INHIBITOR.

Released:
DOI: 10.2210/pdb1gcz/pdb
PDB entry 1gcz coloured by chain, front view.
PDB entry 1gcz coloured by chain, side view.
PDB entry 1gcz coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Coumarin and chromen-4-one analogues as tautomerase inhibitors of macrophage migration inhibitory factor: discovery and X-ray crystallography.
Orita M, Yamamoto S, Katayama N, Aoki M, Takayama K, Yamagiwa Y, Seki N, Suzuki H, Kurihara H, Sakashita H, Takeuchi M, Fujita S, Yamada T, Tanaka A
J Med Chem 44 540-7 (2001)
PMID: 11170644

Function and Biology Details

Reactions catalysed: 
Keto-phenylpyruvate = enol-phenylpyruvate
L-dopachrome = 5,6-dihydroxyindole-2-carboxylate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146863 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Macrophage migration inhibitory factor Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 122 amino acids
Theoretical weight: 13.43 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P14174 (Residues: 2-115; Coverage: 99%)
Gene names: GLIF, MIF, MMIF
Sequence domains: Macrophage migration inhibitory factor (MIF)
Structure domains: Macrophage Migration Inhibitory Factor

Ligands and Environments

3 bound ligands:
Ligand SO4 10 x SO4
Ligand CIT 3 x CIT
Ligand YZ9 3 x YZ9
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-6B
Spacegroup: P212121
Unit cell:
a: 67.6Å b: 67.4Å c: 87.3Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.199 0.199 0.24
Expression system: Escherichia coli BL21

Quick links

Citations

9 review citations

New therapeutic target in inflammatory disease: macrophage migration inhibitory factor.
Morand EF. (2005)
8 more

14 mentions without citation

The marriage of chemokines and galectins as functional heterodimers.
von Hundelshausen et al. (2021)
13 more