› 1gkc

X-ray diffraction
2.3Å resolution

MMP9-inhibitor complex

Released:
DOI: 10.2210/pdb1gkc/pdb
PDB entry 1gkc coloured by chain, front view.
PDB entry 1gkc coloured by chain, side view.
PDB entry 1gkc coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure of human MMP9 in complex with a reverse hydroxamate inhibitor.
Rowsell S, Hawtin P, Minshull CA, Jepson H, Brockbank SM, Barratt DG, Slater AM, McPheat WL, Waterson D, Henney AM, Pauptit RA
J Mol Biol 319 173-81 (2002)
PMID: 12051944

Function and Biology Details

Reaction catalysed: 
Cleavage of gelatin types I and V and collagen types IV and V.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-147085 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
67 kDa matrix metalloproteinase-9 Chains: A, B
Molecule details ›
Chains: A, B
Length: 163 amino acids
Theoretical weight: 18.38 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P14780 (Residues: 107-443; Coverage: 24%)
Gene names: CLG4B, MMP9
Sequence domains: Matrixin
Structure domains: Collagenase (Catalytic Domain)

Ligands and Environments

3 bound ligands:
Ligand CA 10 x CA
Ligand NFH 2 x NFH
Ligand ZN 4 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SRS BEAMLINE PX9.6
Spacegroup: P41212
Unit cell:
a: 56.005Å b: 56.005Å c: 262.655Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.207 0.207 0.239
Expression system: Escherichia coli

Quick links

Citations

16 review citations

Gelatinase-mediated migration and invasion of cancer cells.
Björklund et al. (2005)
15 more

68 mentions without citation

Glycosylation of matrix metalloproteases and tissue inhibitors: present state, challenges and opportunities.
Boon et al. (2016)
67 more