› 1go4

X-ray diffraction
2.05Å resolution

Crystal structure of Mad1-Mad2 reveals a conserved Mad2 binding motif in Mad1 and Cdc20.

Released:
DOI: 10.2210/pdb1go4/pdb
PDB entry 1go4 coloured by chain, front view.
PDB entry 1go4 coloured by chain, side view.
PDB entry 1go4 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure of the tetrameric Mad1-Mad2 core complex: implications of a 'safety belt' binding mechanism for the spindle checkpoint.
Sironi L, Mapelli M, Knapp S, De Antoni A, Jeang KT, Musacchio A
EMBO J 21 2496-506 (2002)
PMID: 12006501

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-171621 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Mitotic spindle assembly checkpoint protein MAD2A Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 205 amino acids
Theoretical weight: 23.45 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q13257 (Residues: 1-205; Coverage: 100%)
Gene names: MAD2, MAD2L1
Sequence domains: HORMA domain
Structure domains: HORMA domain
Mitotic spindle assembly checkpoint protein MAD1 Chains: E, F, G, H
Molecule details ›
Chains: E, F, G, H
Length: 100 amino acids
Theoretical weight: 11.69 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9Y6D9 (Residues: 485-584; Coverage: 14%)
Gene names: MAD1, MAD1L1, TXBP181
Structure domains: Single alpha-helices involved in coiled-coils or other helix-helix interfaces

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF
Spacegroup: P21
Unit cell:
a: 111.037Å b: 63.023Å c: 139.511Å
α: 90° β: 111.65° γ: 90°
R-values:
R R work R free
0.24 0.24 0.268
Expression system: Escherichia coli

Quick links

Citations

45 review citations

The spindle-assembly checkpoint in space and time.
Musacchio et al. (2007)
44 more

21 mentions without citation

BUB1 and BUBR1: multifaceted kinases of the cell cycle.
Bolanos-Garcia et al. (2011)
20 more