› 1gq5

X-ray diffraction
2.2Å resolution

Structural Determinants of the NHERF Interaction with beta2-AR and PDGFR

Released:
DOI: 10.2210/pdb1gq5/pdb
PDB entry 1gq5 coloured by chain, front view.
PDB entry 1gq5 coloured by chain, side view.
PDB entry 1gq5 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural determinants of the Na+/H+ exchanger regulatory factor interaction with the beta 2 adrenergic and platelet-derived growth factor receptors.
Karthikeyan S, Leung T, Ladias JA
J Biol Chem 277 18973-8 (2002)
PMID: 11882663

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-127084 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Na(+)/H(+) exchange regulatory cofactor NHE-RF1 Chain: A
Molecule details ›
Chain: A
Length: 91 amino acids
Theoretical weight: 9.95 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O14745 (Residues: 11-96; Coverage: 24%)
Gene names: NHERF, NHERF1, SLC9A3R1
Sequence domains: PDZ domain
Structure domains: Pdz3 Domain

Ligands and Environments

1 bound ligand:
Ligand CL 2 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU300
Spacegroup: P3121
Unit cell:
a: 50.073Å b: 50.073Å c: 66.68Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.225 0.222 0.263
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

5 review citations

PDZ domains-glue and guide.
van Ham et al. (2003)
4 more

7 mentions without citation

Evaluating caveolin interactions: do proteins interact with the caveolin scaffolding domain through a widespread aromatic residue-rich motif?
Byrne et al. (2012)
6 more