› 1gxc

X-ray diffraction
2.7Å resolution

FHA domain from human Chk2 kinase in complex with a synthetic phosphopeptide

Released:
DOI: 10.2210/pdb1gxc/pdb
PDB entry 1gxc coloured by chain, front view.
PDB entry 1gxc coloured by chain, side view.
PDB entry 1gxc coloured by chain, top view.
Source organisms:
Primary publication:
Structural and functional versatility of the FHA domain in DNA-damage signaling by the tumor suppressor kinase Chk2.
Li J, Williams BL, Haire LF, Goldberg M, Wilker E, Durocher D, Yaffe MB, Jackson SP, Smerdon SJ
Mol Cell 9 1045-54 (2002)
PMID: 12049740

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132132 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Serine/threonine-protein kinase Chk2 Chains: A, D, G, J
Molecule details ›
Chains: A, D, G, J
Length: 149 amino acids
Theoretical weight: 17.44 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O96017 (Residues: 64-212; Coverage: 27%)
Gene names: CDS1, CHEK2, CHK2, RAD53
Sequence domains: FHA domain
Structure domains: Tumour Suppressor Smad4
SYNTHETIC PHOSPHOPEPTIDE Chains: B, E, H, K
Molecule details ›
Chains: B, E, H, K
Length: 10 amino acids
Theoretical weight: 1.46 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

No bound ligands
1 modified residue:
Ligand TPO 4 x TPO

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM14
Spacegroup: P212121
Unit cell:
a: 79.824Å b: 82.884Å c: 129.857Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.232 0.231 0.249
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

28 review citations

Chk1 and Chk2 kinases in checkpoint control and cancer.
Bartek et al. (2003)
27 more

14 mentions without citation

Mechanistic insights into phosphoprotein-binding FHA domains.
Liang et al. (2008)
13 more