› 1h0d

X-ray diffraction
2Å resolution

Crystal structure of Human Angiogenin in complex with Fab fragment of its monoclonal antibody mAb 26-2F

Released:
DOI: 10.2210/pdb1h0d/pdb
PDB entry 1h0d coloured by chain, front view.
PDB entry 1h0d coloured by chain, side view.
PDB entry 1h0d coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The crystal structure of human angiogenin in complex with an antitumor neutralizing antibody.
Chavali GB, Papageorgiou AC, Olson KA, Fett JW, Hu Gf, Shapiro R, Acharya KR
Structure 11 875-85 (2003)
PMID: 12842050

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-208967 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
ANTIBODY FAB FRAGMENT, LIGHT CHAIN Chain: A
Molecule details ›
Chain: A
Length: 216 amino acids
Theoretical weight: 23.58 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Structure domains: Immunoglobulins
ANTIBODY FAB FRAGMENT, HEAVY CHAIN Chain: B
Molecule details ›
Chain: B
Length: 223 amino acids
Theoretical weight: 23.66 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Structure domains: Immunoglobulins
Angiogenin Chain: C
Molecule details ›
Chain: C
Length: 123 amino acids
Theoretical weight: 14.15 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P03950 (Residues: 25-147; Coverage: 100%)
Gene names: ANG, RNASE5
Sequence domains: Pancreatic ribonuclease
Structure domains: Ribonuclease A-like domain

Ligands and Environments

2 bound ligands:
Ligand SO4 8 x SO4
Ligand GOL 6 x GOL
1 modified residue:
Ligand PCA 1 x PCA

Experiments and Validation Details

Entry percentile scores
X-ray source: SRS BEAMLINE PX9.6
Spacegroup: C2
Unit cell:
a: 120.58Å b: 72.53Å c: 86.99Å
α: 90° β: 112.46° γ: 90°
R-values:
R R work R free
0.232 0.232 0.272
Expression system: Escherichia coli

Quick links

Citations

13 citation in other articles

Angiogenin loss-of-function mutations in amyotrophic lateral sclerosis.
Wu et al. (2007)
12 more

13 mentions without citation

Immune epitope database analysis resource (IEDB-AR).
Zhang et al. (2008)
12 more