› 1h15

X-ray diffraction
3.1Å resolution

X-ray crystal structure of HLA-DRA1*0101/DRB5*0101 complexed with a peptide from Epstein Barr Virus DNA polymerase

Released:
DOI: 10.2210/pdb1h15/pdb
PDB entry 1h15 coloured by chain, front view.
PDB entry 1h15 coloured by chain, side view.
PDB entry 1h15 coloured by chain, top view.
Source organisms:
Primary publication:
A functional and structural basis for TCR cross-reactivity in multiple sclerosis.
Lang HL, Jacobsen H, Ikemizu S, Andersson C, Harlos K, Madsen L, Hjorth P, Sondergaard L, Svejgaard A, Wucherpfennig K, Stuart DI, Bell JI, Jones EY, Fugger L
Nat Immunol 3 940-3 (2002)
PMID: 12244309

Function and Biology Details

Reaction catalysed: 
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-135351 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (4 distinct):
HLA class II histocompatibility antigen, DR alpha chain Chains: A, D
Molecule details ›
Chains: A, D
Length: 182 amino acids
Theoretical weight: 21.16 KDa
Source organism: Homo sapiens
Expression system: Drosophila melanogaster
UniProt:
  • Canonical: P01903 (Residues: 26-207; Coverage: 80%)
Gene names: HLA-DRA, HLA-DRA1
Sequence domains:
Structure domains:
HLA class II histocompatibility antigen, DR beta 5 chain Chains: B, E
Molecule details ›
Chains: B, E
Length: 190 amino acids
Theoretical weight: 22.23 KDa
Source organism: Homo sapiens
Expression system: Drosophila melanogaster
UniProt:
  • Canonical: Q30154 (Residues: 30-219; Coverage: 80%)
Gene name: HLA-DRB5
Sequence domains:
Structure domains:
DNA polymerase catalytic subunit Chains: C, F
Molecule details ›
Chains: C, F
Length: 14 amino acids
Theoretical weight: 1.63 KDa
Source organism: human gammaherpesvirus 4
Expression system: Not provided
UniProt:
  • Canonical: P03198 (Residues: 628-641; Coverage: 1%)
Gene name: BALF5

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
1 bound ligand:
Ligand NAG 3 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P65
Unit cell:
a: 179.244Å b: 179.244Å c: 92.884Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.256 0.256 0.31
Expression systems:
  • Drosophila melanogaster
  • Not provided

Quick links

Citations

148 review citations

Mechanisms underlying inflammation in neurodegeneration.
Glass et al. (2010)
147 more

49 mentions without citation

Conformational variation in structures of classical and non-classical MHCII proteins and functional implications.
Painter et al. (2012)
48 more