› 1h4w

X-ray diffraction
1.7Å resolution

Structure of human trypsin IV (brain trypsin)

Released:
DOI: 10.2210/pdb1h4w/pdb
PDB entry 1h4w coloured by chain, front view.
PDB entry 1h4w coloured by chain, side view.
PDB entry 1h4w coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure reveals basis for the inhibitor resistance of human brain trypsin.
Katona G, Berglund GI, Hajdu J, Gráf L, Szilágyi L
J Mol Biol 315 1209-18 (2002)
PMID: 11827488

Function and Biology Details

Reaction catalysed: 
Preferential cleavage: Arg-|-, Lys-|-.
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152772 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Trypsin-3 Chain: A
Molecule details ›
Chain: A
Length: 224 amino acids
Theoretical weight: 24.3 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P35030 (Residues: 81-304; Coverage: 74%)
Gene names: PRSS3, PRSS4, TRY3, TRY4
Sequence domains: Trypsin
Structure domains: Trypsin-like serine proteases

Ligands and Environments

2 bound ligands:
Ligand BEN 1 x BEN
Ligand CA 1 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM14
Spacegroup: P41212
Unit cell:
a: 56.683Å b: 56.683Å c: 143.21Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.188 0.188 0.203
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

6 review citations

Protease-activated receptors: contribution to physiology and disease.
Ossovskaya et al. (2004)
5 more

23 mentions without citation

Targeting PAI-1 in Cardiovascular Disease: Structural Insights Into PAI-1 Functionality and Inhibition.
Sillen et al. (2020)
22 more