› 1h6e

X-ray diffraction
3.6Å resolution

MU2 ADAPTIN SUBUNIT (AP50) OF AP2 ADAPTOR (SECOND DOMAIN), COMPLEXED WITH CTLA-4 INTERNALIZATION PEPTIDE TTGVYVKMPPT

Released:
DOI: 10.2210/pdb1h6e/pdb
PDB entry 1h6e coloured by chain, front view.
PDB entry 1h6e coloured by chain, side view.
PDB entry 1h6e coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Study of the interaction of the medium chain mu 2 subunit of the clathrin-associated adapter protein complex 2 with cytotoxic T-lymphocyte antigen 4 and CD28.
Follows ER, McPheat JC, Minshull C, Moore NC, Pauptit RA, Rowsell S, Stacey CL, Stanway JJ, Taylor IW, Abbott WM
Biochem J 359 427-34 (2001)
PMID: 11583591

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-147705 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
AP-2 complex subunit mu Chain: A
Molecule details ›
Chain: A
Length: 288 amino acids
Theoretical weight: 33.01 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q96CW1 (Residues: 141-435; Coverage: 66%)
Gene names: AP2M1, CLAPM1, KIAA0109
Sequence domains: Adaptor complexes medium subunit family
Structure domains: Mu homology domain, subdomain B
Cytotoxic T-lymphocyte protein 4 Chain: P
Molecule details ›
Chain: P
Length: 11 amino acids
Theoretical weight: 1.19 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P16410 (Residues: 197-207; Coverage: 6%)
Gene names: CD152, CTLA4

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SRS BEAMLINE PX9.6
Spacegroup: P64
Unit cell:
a: 126.779Å b: 126.779Å c: 74.161Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.278 0.278 0.317
Expression systems:
  • Escherichia coli BL21
  • Not provided

Quick links

Citations

4 review citations

Adaptors for clathrin coats: structure and function.
Owen et al. (2004)
3 more

1 mention without citation

Crystal structure of the clathrin adaptor protein 1 core.
Heldwein et al. (2004)