› 1hcq

X-ray diffraction
2.4Å resolution

THE CRYSTAL STRUCTURE OF THE ESTROGEN RECEPTOR DNA-BINDING DOMAIN BOUND TO DNA: HOW RECEPTORS DISCRIMINATE BETWEEN THEIR RESPONSE ELEMENTS

Released:
DOI: 10.2210/pdb1hcq/pdb
PDB entry 1hcq coloured by chain, front view.
PDB entry 1hcq coloured by chain, side view.
PDB entry 1hcq coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The crystal structure of the estrogen receptor DNA-binding domain bound to DNA: how receptors discriminate between their response elements.
Schwabe JW, Chapman L, Finch JT, Rhodes D
Cell 75 567-78 (1993)
PMID: 8221895

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-113893 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Estrogen receptor Chains: A, B, E, F
Molecule details ›
Chains: A, B, E, F
Length: 84 amino acids
Theoretical weight: 9.71 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P03372 (Residues: 180-262; Coverage: 14%)
Gene names: ESR, ESR1, NR3A1
Sequence domains: Zinc finger, C4 type (two domains)
Structure domains: Erythroid Transcription Factor GATA-1, subunit A
DNA (5'-D(*CP*CP*AP*GP*GP*TP*CP*AP*CP*AP*GP*TP*GP*AP*CP*CP*T P*G)-3') Chains: C, G
Molecule details ›
Chains: C, G
Length: 18 nucleotides
Theoretical weight: 5.5 KDa
Source organism: Homo sapiens
Expression system: Not provided
DNA (5'-D(*CP*CP*AP*GP*GP*TP*CP*AP*CP*TP*GP*TP*GP*AP*CP*CP*T P*G)-3') Chains: D, H
Molecule details ›
Chains: D, H
Length: 18 nucleotides
Theoretical weight: 5.49 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand ZN 8 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P212121
Unit cell:
a: 51.6Å b: 90.8Å c: 114.6Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.204 0.204 not available
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

102 review citations

The RXR heterodimers and orphan receptors.
Mangelsdorf et al. (1995)
101 more

51 mentions without citation

Molecular mechanism of estrogen-estrogen receptor signaling.
Yaşar et al. (2017)
50 more