› 1hei

X-ray diffraction
2.1Å resolution

STRUCTURE OF THE HEPATITIS C VIRUS RNA HELICASE DOMAIN

Released:
DOI: 10.2210/pdb1hei/pdb
PDB entry 1hei coloured by chain, front view.
PDB entry 1hei coloured by chain, side view.
PDB entry 1hei coloured by chain, top view.
Source organism: Hepatitis C virus (isolate 1)
Primary publication:
Structure of the hepatitis C virus RNA helicase domain.
Yao N, Hesson T, Cable M, Hong Z, Kwong AD, Le HV, Weber PC
Nat Struct Biol 4 463-7 (1997)
PMID: 9187654

Function and Biology Details

Reactions catalysed: 
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Hydrolysis of four peptide bonds in the viral precursor polyprotein, commonly with Asp or Glu in the P6 position, Cys or Thr in P1 and Ser or Ala in P1'.
NTP + H(2)O = NDP + phosphate
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151128 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Serine protease/helicase NS3 Chains: A, B
Molecule details ›
Chains: A, B
Length: 451 amino acids
Theoretical weight: 48.36 KDa
Source organism: Hepatitis C virus (isolate 1)
Expression system: Escherichia coli
UniProt:
  • Canonical: P27958 (Residues: 1206-1656; Coverage: 15%)
Sequence domains: Flavivirus DEAD domain
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand CA 2 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH2R
Spacegroup: P212121
Unit cell:
a: 81.54Å b: 102.73Å c: 119.5Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.22 0.22 0.32
Expression system: Escherichia coli

Quick links

Citations

68 review citations

Mechanical devices of the spliceosome: motors, clocks, springs, and things.
Staley et al. (1998)
67 more

18 mentions without citation

Viral and cellular RNA helicases as antiviral targets.
Kwong et al. (2005)
17 more