› 1hg4

X-ray diffraction
2.4Å resolution

Ultraspiracle ligand binding domain from Drosophila melanogaster

Released:
DOI: 10.2210/pdb1hg4/pdb
PDB entry 1hg4 coloured by chain, front view.
PDB entry 1hg4 coloured by chain, side view.
PDB entry 1hg4 coloured by chain, top view.
Source organism: Drosophila melanogaster
Primary publication:
The structure of the ultraspiracle ligand-binding domain reveals a nuclear receptor locked in an inactive conformation.
Clayton GM, Peak-Chew SY, Evans RM, Schwabe JW
Proc Natl Acad Sci U S A 98 1549-54 (2001)
PMID: 11171988

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-148862 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Protein ultraspiracle Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 279 amino acids
Theoretical weight: 31.36 KDa
Source organism: Drosophila melanogaster
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P20153 (Residues: 230-508; Coverage: 55%)
Gene names: CG4380, Cf1, NR2B4, usp
Sequence domains: Ligand-binding domain of nuclear hormone receptor
Structure domains: Retinoid X Receptor

Ligands and Environments

1 bound ligand:
Ligand LPP 6 x LPP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SRS BEAMLINE PX9.6
Spacegroup: P1
Unit cell:
a: 37.98Å b: 86.19Å c: 137Å
α: 85.57° β: 85.94° γ: 83.09°
R-values:
R R work R free
0.248 0.248 0.284
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

14 review citations

The juvenile hormone signaling pathway in insect development.
Jindra et al. (2013)
13 more

5 mentions without citation

The retinoid X receptors and their ligands.
Dawson et al. (2012)
4 more