› 1hi3

X-ray diffraction
1.8Å resolution

Eosinophil-derived Neurotoxin (EDN) - Adenosine 2'-5'-Diphosphate Complex

Released:
DOI: 10.2210/pdb1hi3/pdb
PDB entry 1hi3 coloured by chain, front view.
PDB entry 1hi3 coloured by chain, side view.
PDB entry 1hi3 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Mapping the ribonucleolytic active site of eosinophil-derived neurotoxin (EDN). High resolution crystal structures of EDN complexes with adenylic nucleotide inhibitors.
Leonidas DD, Boix E, Prill R, Suzuki M, Turton R, Minson K, Swaminathan GJ, Youle RJ, Acharya KR
J Biol Chem 276 15009-17 (2001)
PMID: 11154698

Function and Biology Details

Reaction catalysed: 
(1a) an (RNA) containing cytidine = an (RNA)-3'-cytidine-2',3'-cyclophosphate + a 5'-hydroxy-ribonucleotide-3'-(RNA)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-145194 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Non-secretory ribonuclease Chain: A
Molecule details ›
Chain: A
Length: 135 amino acids
Theoretical weight: 15.61 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P10153 (Residues: 28-161; Coverage: 100%)
Gene names: EDN, RNASE2, RNS2
Sequence domains: Pancreatic ribonuclease
Structure domains: Ribonuclease A-like domain

Ligands and Environments

1 bound ligand:
Ligand A2P 1 x A2P
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ENRAF-NONIUS
Spacegroup: P212121
Unit cell:
a: 52.525Å b: 56.176Å c: 41.473Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.193 0.193 0.259
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Eosinophil granule proteins: form and function.
Acharya et al. (2014)
3 more

3 mentions without citation

High-resolution crystal structures of ribonuclease A complexed with adenylic and uridylic nucleotide inhibitors. Implications for structure-based design of ribonucleolytic inhibitors.
Leonidas et al. (2003)
2 more