› 1hjb

X-ray diffraction
3Å resolution

CRYSTAL STRUCTURE OF RUNX-1/AML1/CBFALPHA RUNT DOMAIN AND C/EBPBETA BZIP HOMODIMER BOUND TO A DNA FRAGMENT FROM THE CSF-1R PROMOTER

Released:
DOI: 10.2210/pdb1hjb/pdb
PDB entry 1hjb coloured by chain, front view.
PDB entry 1hjb coloured by chain, side view.
PDB entry 1hjb coloured by chain, top view.
Source organisms:
Primary publication:
Structural analyses of DNA recognition by the AML1/Runx-1 Runt domain and its allosteric control by CBFbeta.
Tahirov TH, Inoue-Bungo T, Morii H, Fujikawa A, Sasaki M, Kimura K, Shiina M, Sato K, Kumasaka T, Yamamoto M, Ishii S, Ogata K
Cell 104 755-67 (2001)
PMID: 11257229

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-113900 (preferred)
Entry contents:
2 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (4 distinct):
CCAAT/enhancer-binding protein beta Chains: A, B, D, E
Molecule details ›
Chains: A, B, D, E
Length: 87 amino acids
Theoretical weight: 10.25 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P17676 (Residues: 259-345; Coverage: 25%)
Gene names: CEBPB, PP9092, TCF5
Sequence domains: Basic region leucine zipper
Structure domains: Single alpha-helices involved in coiled-coils or other helix-helix interfaces
Runt-related transcription factor 1 Chains: C, F
Molecule details ›
Chains: C, F
Length: 123 amino acids
Theoretical weight: 13.72 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q03347 (Residues: 60-182; Coverage: 27%)
Gene names: Aml1, Cbfa2, Pebp2ab, Runx1
Sequence domains: Runt domain
Structure domains: Immunoglobulin-like
DNA (5'-(*GP*AP*AP*GP*AP*TP*TP*TP*CP*CP* AP*AP*AP*CP*TP*CP*TP*GP*TP*GP*GP*TP*TP*GP*CP*G)-3') Chains: G, I
Molecule details ›
Chains: G, I
Length: 26 nucleotides
Theoretical weight: 8.02 KDa
DNA (5'-(*CP*CP*GP*CP*AP*AP*CP*CP*AP*CP* AP*GP*AP*GP*TP*TP*TP*GP*GP*AP*AP*AP*TP*CP*TP*T)-3') Chains: H, J
Molecule details ›
Chains: H, J
Length: 26 nucleotides
Theoretical weight: 7.96 KDa

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL41XU
Spacegroup: P21212
Unit cell:
a: 102.165Å b: 109.273Å c: 127.405Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.244 0.244 0.313
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

56 review citations

CCAAT/enhancer-binding proteins: structure, function and regulation.
Ramji et al. (2002)
55 more

7 mentions without citation

Overview of protein structural and functional folds.
Sun et al. (2004)
6 more