› 1hlg

X-ray diffraction
3Å resolution

CRYSTAL STRUCTURE OF HUMAN GASTRIC LIPASE

Released:
DOI: 10.2210/pdb1hlg/pdb
PDB entry 1hlg coloured by chain, front view.
PDB entry 1hlg coloured by chain, side view.
PDB entry 1hlg coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure of human gastric lipase and model of lysosomal acid lipase, two lipolytic enzymes of medical interest.
Roussel A, Canaan S, Egloff MP, Rivière M, Dupuis L, Verger R, Cambillau C
J Biol Chem 274 16995-7002 (1999)
PMID: 10358049

Function and Biology Details

Reaction catalysed: 
Triacylglycerol + H(2)O = diacylglycerol + a carboxylate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139370 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Gastric triacylglycerol lipase Chains: A, B
Molecule details ›
Chains: A, B
Length: 371 amino acids
Theoretical weight: 42.4 KDa
Source organism: Homo sapiens
Expression system: INSECT CELL
UniProt:
  • Canonical: P07098 (Residues: 28-398; Coverage: 98%)
Gene name: LIPF
Sequence domains: alpha/beta hydrolase fold
Structure domains: alpha/beta hydrolase

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
1 bound ligand:
Ligand NAG 6 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: LURE BEAMLINE DW32
Spacegroup: I213
Unit cell:
a: 244.33Å b: 244.33Å c: 244.33Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.21 0.21 0.228
Expression system: INSECT CELL

Quick links

Citations

16 review citations

Alpha/beta hydrolase fold enzymes: the family keeps growing.
Nardini et al. (1999)
15 more

15 mentions without citation

Deamidation of human proteins.
Robinson et al. (2001)
14 more