› 1hlo

X-ray diffraction
2.8Å resolution

THE CRYSTAL STRUCTURE OF AN INTACT HUMAN MAX-DNA COMPLEX: NEW INSIGHTS INTO MECHANISMS OF TRANSCRIPTIONAL CONTROL

Released:
DOI: 10.2210/pdb1hlo/pdb
PDB entry 1hlo coloured by chain, front view.
PDB entry 1hlo coloured by chain, side view.
PDB entry 1hlo coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The crystal structure of an intact human Max-DNA complex: new insights into mechanisms of transcriptional control.
Brownlie P, Ceska T, Lamers M, Romier C, Stier G, Teo H, Suck D
Structure 5 509-20 (1997)
PMID: 9115440

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-113902 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Protein max Chains: A, B
Molecule details ›
Chains: A, B
Length: 80 amino acids
Theoretical weight: 9.54 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P61244 (Residues: 4-92; Coverage: 50%)
Gene names: BHLHD4, MAX
Sequence domains: Helix-loop-helix DNA-binding domain
Structure domains: Helix-loop-helix DNA-binding domain
DNA (5'-D(*CP*AP*CP*CP*AP*CP*GP*TP*GP*GP*T)-3') Chain: C
Molecule details ›
Chain: C
Length: 11 nucleotides
Theoretical weight: 3.33 KDa
Source organism: Homo sapiens
Expression system: Not provided
DNA (5'-D(*AP*CP*CP*AP*CP*GP*TP*GP*GP*TP*G)-3') Chain: D
Molecule details ›
Chain: D
Length: 11 nucleotides
Theoretical weight: 3.37 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P6522
Unit cell:
a: 108Å b: 108Å c: 127Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.213 0.213 0.273
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

11 review citations

The Myc/Max/Mad network and the transcriptional control of cell behavior.
Grandori et al. (2000)
10 more

17 mentions without citation

An overview of the basic helix-loop-helix proteins.
Jones S. (2004)
16 more