› 1hne

X-ray diffraction
1.84Å resolution

Structure of human neutrophil elastase in complex with a peptide chloromethyl ketone inhibitor at 1.84-angstroms resolution

Released:
DOI: 10.2210/pdb1hne/pdb
PDB entry 1hne coloured by chain, front view.
PDB entry 1hne coloured by chain, side view.
PDB entry 1hne coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure of human neutrophil elastase in complex with a peptide chloromethyl ketone inhibitor at 1.84-A resolution.
Navia MA, McKeever BM, Springer JP, Lin TY, Williams HR, Fluder EM, Dorn CP, Hoogsteen K
Proc Natl Acad Sci U S A 86 7-11 (1989)
PMID: 2911584

Function and Biology Details

Reaction catalysed: 
Hydrolysis of proteins, including elastin. Preferential cleavage Val-|- > Ala-|-.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140071 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Neutrophil elastase Chain: E
Molecule details ›
Chain: E
Length: 218 amino acids
Theoretical weight: 23.32 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P08246 (Residues: 30-247; Coverage: 91%)
Gene names: ELA2, ELANE
Sequence domains: Trypsin
Structure domains: Trypsin-like serine proteases
METHOXYSUCCINYL-ALA-ALA-PRO-ALA CHLOROMETHYL KETONE INHIBITOR Chain: I
Molecule details ›
Chain: I
Length: 6 amino acids
Theoretical weight: 477 Da
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P63
Unit cell:
a: 74.53Å b: 74.53Å c: 70.88Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.164 not available not available
Expression system: Not provided

Quick links

Citations

15 review citations

Structural basis of substrate specificity in the serine proteases.
Perona et al. (1995)
14 more

29 mentions without citation

Heparan sulfate-protein binding specificity.
Nugent et al. (2013)
28 more