› 1hsz

X-ray diffraction
2.2Å resolution

HUMAN BETA-1 ALCOHOL DEHYDROGENASE (ADH1B*1)

Released:
DOI: 10.2210/pdb1hsz/pdb
PDB entry 1hsz coloured by chain, front view.
PDB entry 1hsz coloured by chain, side view.
PDB entry 1hsz coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Three-dimensional structures of the three human class I alcohol dehydrogenases.
Niederhut MS, Gibbons BJ, Perez-Miller S, Hurley TD
Protein Sci 10 697-706 (2001)
PMID: 11274460

Function and Biology Details

Reaction catalysed: 
All-trans-retinol-[cellular-retinol-binding-protein] + NAD(+) = all-trans-retinal-[cellular-retinol-binding-protein] + NADH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132402 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
All-trans-retinol dehydrogenase [NAD(+)] ADH1B Chains: A, B
Molecule details ›
Chains: A, B
Length: 374 amino acids
Theoretical weight: 39.77 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P00325 (Residues: 2-375; Coverage: 100%)
Gene names: ADH1B, ADH2
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand NAD 2 x NAD
2 bound ligands:
Ligand ZN 4 x ZN
Ligand PO4 1 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P1
Unit cell:
a: 43.9Å b: 53.4Å c: 90.2Å
α: 79.9° β: 89.5° γ: 69.3°
R-values:
R R work R free
0.194 0.193 0.257
Expression system: Escherichia coli

Quick links

Citations

11 review citations

Effect of the allelic variants of aldehyde dehydrogenase ALDH2*2 and alcohol dehydrogenase ADH1B*2 on blood acetaldehyde concentrations.
Peng et al. (2009)
10 more

6 mentions without citation

Three-dimensional structures of the three human class I alcohol dehydrogenases.
Niederhut et al. (2001)
5 more