› 1htj

X-ray diffraction
2.2Å resolution

STRUCTURE OF THE RGS-LIKE DOMAIN FROM PDZ-RHOGEF

Released:
DOI: 10.2210/pdb1htj/pdb
PDB entry 1htj coloured by chain, front view.
PDB entry 1htj coloured by chain, side view.
PDB entry 1htj coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure of the RGS-like domain from PDZ-RhoGEF: linking heterotrimeric g protein-coupled signaling to Rho GTPases.
Longenecker KL, Lewis ME, Chikumi H, Gutkind JS, Derewenda ZS
Structure 9 559-69 (2001)
PMID: 11470431

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-127284 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Rho guanine nucleotide exchange factor 11 Chain: F
Molecule details ›
Chain: F
Length: 210 amino acids
Theoretical weight: 23.9 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O15085 (Residues: 281-490; Coverage: 14%)
Gene names: ARHGEF11, KIAA0380
Sequence domains: Regulator of G protein signalling-like domain
Structure domains: Regulator of G-protein Signalling 4, domain 2

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 4 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X9B
Spacegroup: P6122
Unit cell:
a: 61.61Å b: 61.61Å c: 201.91Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.226 0.223 0.258
Expression system: Escherichia coli

Quick links

Citations

10 review citations

Ca2+ sensitivity of smooth muscle and nonmuscle myosin II: modulated by G proteins, kinases, and myosin phosphatase.
Somlyo et al. (2003)
9 more

7 mentions without citation

Structure and function of heterotrimeric G protein-regulated Rho guanine nucleotide exchange factors.
Aittaleb et al. (2010)
6 more