› 1i00

X-ray diffraction
2.5Å resolution

CRYSTAL STRUCTURE OF HUMAN THYMIDYLATE SYNTHASE, TERNARY COMPLEX WITH DUMP AND TOMUDEX

Released:
DOI: 10.2210/pdb1i00/pdb
PDB entry 1i00 coloured by chain, front view.
PDB entry 1i00 coloured by chain, side view.
PDB entry 1i00 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure of a deletion mutant of human thymidylate synthase Delta (7-29) and its ternary complex with Tomudex and dUMP.
Almog R, Waddling CA, Maley F, Maley GF, Van Roey P
Protein Sci 10 988-96 (2001)
PMID: 11316879

Function and Biology Details

Reaction catalysed: 
5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-138196 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Thymidylate synthase Chains: A, B
Molecule details ›
Chains: A, B
Length: 290 amino acids
Theoretical weight: 33.19 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P04818 (Residues: 26-29, 30-313; Coverage: 92%)
Gene names: OK/SW-cl.29, TS, TYMS
Sequence domains: Thymidylate synthase
Structure domains: Thymidylate synthase/dCMP hydroxymethylase domain

Ligands and Environments

2 bound ligands:
Ligand UMP 2 x UMP
Ligand D16 2 x D16
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P3121
Unit cell:
a: 89.689Å b: 89.689Å c: 142.129Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.207 0.207 0.262
Expression system: Escherichia coli

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Citations

3 review citations

Ubiquitin-independent degradation of proteins by the proteasome.
Jariel-Encontre et al. (2008)
2 more

13 mentions without citation

Crystal structure of a deletion mutant of human thymidylate synthase Delta (7-29) and its ternary complex with Tomudex and dUMP.
Almog et al. (2001)
12 more