› 1i27

X-ray diffraction
1.02Å resolution

CRYSTAL STRUCTURE OF THE C-TERMINAL DOMAIN OF THE RAP74 SUBUNIT OF HUMAN TRANSCRIPTION FACTOR IIF (TFIIF)

Released:
DOI: 10.2210/pdb1i27/pdb
PDB entry 1i27 coloured by chain, front view.
PDB entry 1i27 coloured by chain, side view.
PDB entry 1i27 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure of the C-terminal domain of the RAP74 subunit of human transcription factor IIF.
Kamada K, De Angelis J, Roeder RG, Burley SK
Proc Natl Acad Sci U S A 98 3115-20 (2001)
PMID: 11248041

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152877 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
General transcription factor IIF subunit 1 Chain: A
Molecule details ›
Chain: A
Length: 73 amino acids
Theoretical weight: 8.37 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P35269 (Residues: 449-517; Coverage: 13%)
Gene names: GTF2F1, RAP74
Structure domains: Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain

Ligands and Environments

1 bound ligand:
Ligand ZN 1 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P212121
Unit cell:
a: 29.052Å b: 43.024Å c: 48.161Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.127 not available 0.146
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

7 review citations

The general transcription machinery and general cofactors.
Thomas et al. (2006)
6 more

21 mentions without citation

Optimization of the additive CHARMM all-atom protein force field targeting improved sampling of the backbone φ, ψ and side-chain χ(1) and χ(2) dihedral angles.
Best et al. (2012)
20 more