› 1i44

X-ray diffraction
2.4Å resolution

CRYSTALLOGRAPHIC STUDIES OF AN ACTIVATION LOOP MUTANT OF THE INSULIN RECEPTOR TYROSINE KINASE

Released:
DOI: 10.2210/pdb1i44/pdb
PDB entry 1i44 coloured by chain, front view.
PDB entry 1i44 coloured by chain, side view.
PDB entry 1i44 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystallographic and solution studies of an activation loop mutant of the insulin receptor tyrosine kinase: insights into kinase mechanism.
Till JH, Ablooglu AJ, Frankel M, Bishop SM, Kohanski RA, Hubbard SR
J Biol Chem 276 10049-55 (2001)
PMID: 11124964

Function and Biology Details

Reaction catalysed: 
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-138931 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Insulin receptor subunit beta Chain: A
Molecule details ›
Chain: A
Length: 306 amino acids
Theoretical weight: 34.75 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P06213 (Residues: 1005-1310; Coverage: 23%)
Gene name: INSR
Sequence domains: Protein tyrosine and serine/threonine kinase
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand MG 1 x MG
Ligand ACP 1 x ACP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P212121
Unit cell:
a: 57.857Å b: 69.578Å c: 89.26Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.21 0.21 0.268
Expression system: Spodoptera frugiperda

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Citations

9 review citations

FLT3: ITDoes matter in leukemia.
Levis et al. (2003)
8 more

2 mentions without citation

Signal transduction in histidine kinases: insights from new structures.
Bhate et al. (2015)
1 more