› 1ial

X-ray diffraction
2.5Å resolution

IMPORTIN ALPHA, MOUSE

Released:
DOI: 10.2210/pdb1ial/pdb
PDB entry 1ial coloured by chain, front view.
PDB entry 1ial coloured by chain, side view.
PDB entry 1ial coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Autoinhibition by an internal nuclear localization signal revealed by the crystal structure of mammalian importin alpha.
Kobe B
Nat Struct Biol 6 388-97 (1999)
PMID: 10201409

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156499 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Importin subunit alpha-1 Chain: A
Molecule details ›
Chain: A
Length: 453 amino acids
Theoretical weight: 49.33 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P52293 (Residues: 44-496; Coverage: 86%)
Gene names: Kpna2, Rch1
Sequence domains:
Structure domains: Leucine-rich Repeat Variant

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH2R
Spacegroup: P212121
Unit cell:
a: 78.549Å b: 89.675Å c: 100.562Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.189 0.189 0.24
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

36 review citations

Transport between the cell nucleus and the cytoplasm.
Görlich et al. (1999)
35 more

18 mentions without citation

Recognition of nuclear targeting signals by Karyopherin-β proteins.
Xu et al. (2010)
17 more