› 1iat

X-ray diffraction
1.62Å resolution

CRYSTAL STRUCTURE OF HUMAN PHOSPHOGLUCOSE ISOMERASE/NEUROLEUKIN/AUTOCRINE MOTILITY FACTOR/MATURATION FACTOR

Released:
DOI: 10.2210/pdb1iat/pdb
PDB entry 1iat coloured by chain, front view.
PDB entry 1iat coloured by chain, side view.
PDB entry 1iat coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The crystal structure of human phosphoglucose isomerase at 1.6 A resolution: implications for catalytic mechanism, cytokine activity and haemolytic anaemia.
Read J, Pearce J, Li X, Muirhead H, Chirgwin J, Davies C
J Mol Biol 309 447-63 (2001)
PMID: 11371164

Function and Biology Details

Reaction catalysed: 
Alpha-D-glucose 6-phosphate = beta-D-fructofuranose 6-phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139198 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glucose-6-phosphate isomerase Chain: A
Molecule details ›
Chain: A
Length: 557 amino acids
Theoretical weight: 63.1 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P06744 (Residues: 2-558; Coverage: 100%)
Gene name: GPI
Sequence domains: Phosphoglucose isomerase
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand SO4 2 x SO4
Ligand BME 1 x BME
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SRS BEAMLINE PX7.2
Spacegroup: P43212
Unit cell:
a: 94.183Å b: 94.183Å c: 136.138Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.147 0.144 0.17
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Carbohydrate metabolism in Archaea: current insights into unusual enzymes and pathways and their regulation.
Bräsen et al. (2014)
3 more

6 mentions without citation

From DNA to fitness differences: sequences and structures of adaptive variants of Colias phosphoglucose isomerase (PGI).
Wheat et al. (2006)
5 more