› 1im9

X-ray diffraction
2.8Å resolution

Crystal structure of the human natural killer cell inhibitory receptor KIR2DL1 bound to its MHC ligand HLA-Cw4

Released:
DOI: 10.2210/pdb1im9/pdb
PDB entry 1im9 coloured by chain, front view.
PDB entry 1im9 coloured by chain, side view.
PDB entry 1im9 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure of the human natural killer cell inhibitory receptor KIR2DL1-HLA-Cw4 complex.
Fan QR, Long EO, Wiley DC
Nat Immunol 2 452-60 (2001)
PMID: 11323700

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero heptamer (preferred)
PDBe Complex ID:
PDB-CPX-145278 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
HLA class I histocompatibility antigen, C alpha chain Chains: A, E
Molecule details ›
Chains: A, E
Length: 276 amino acids
Theoretical weight: 32.15 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P10321 (Residues: 26-299; Coverage: 80%)
Gene names: HLA-C, HLAC
Sequence domains:
Structure domains:
Beta-2-microglobulin Chains: B, F
Molecule details ›
Chains: B, F
Length: 100 amino acids
Theoretical weight: 11.88 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P61769 (Residues: 21-119; Coverage: 100%)
Gene names: B2M, CDABP0092, HDCMA22P
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins
HLA-Cw4-specific peptide Chains: C, G
Molecule details ›
Chains: C, G
Length: 9 amino acids
Theoretical weight: 1.12 KDa
Killer cell immunoglobulin-like receptor 2DL1 Chain: D
Molecule details ›
Chain: D
Length: 225 amino acids
Theoretical weight: 24.77 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P43626 (Residues: 22-245; Coverage: 69%)
Gene names: CD158A, KIR2DL1, NKAT1
Sequence domains: Immunoglobulin domain
Structure domains: Immunoglobulins

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: C2
Unit cell:
a: 213.631Å b: 75.867Å c: 125.746Å
α: 90° β: 120.96° γ: 90°
R-values:
R R work R free
0.218 0.213 0.256
Expression system: Escherichia coli

Quick links

Citations

47 review citations

KIR: diverse, rapidly evolving receptors of innate and adaptive immunity.
Vilches et al. (2002)
46 more

32 mentions without citation

Structural basis for recognition of MHC and MHC-like ligands by natural killer cell receptors.
Deng et al. (2006)
31 more