› 1j1e

X-ray diffraction
3.3Å resolution

Crystal structure of the 52kDa domain of human cardiac troponin in the Ca2+ saturated form

Released:
DOI: 10.2210/pdb1j1e/pdb
PDB entry 1j1e coloured by chain, front view.
PDB entry 1j1e coloured by chain, side view.
PDB entry 1j1e coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure of the core domain of human cardiac troponin in the Ca(2+)-saturated form.
Takeda S, Yamashita A, Maeda K, Maéda Y
Nature 424 35-41 (2003)
PMID: 12840750

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-148573 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Troponin C, slow skeletal and cardiac muscles Chains: A, D
Molecule details ›
Chains: A, D
Length: 161 amino acids
Theoretical weight: 18.4 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P63316 (Residues: 1-161; Coverage: 100%)
Gene names: TNNC, TNNC1
Sequence domains:
Structure domains: EF-hand
Troponin T, cardiac muscle Chains: B, E
Molecule details ›
Chains: B, E
Length: 106 amino acids
Theoretical weight: 12.84 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P45379 (Residues: 193-298; Coverage: 36%)
Gene name: TNNT2
Sequence domains: Troponin
Structure domains: Single alpha-helices involved in coiled-coils or other helix-helix interfaces
Troponin I, cardiac muscle Chains: C, F
Molecule details ›
Chains: C, F
Length: 180 amino acids
Theoretical weight: 20.75 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P19429 (Residues: 31-210; Coverage: 86%)
Gene names: TNNC1, TNNI3
Sequence domains: Troponin
Structure domains: Single alpha-helices involved in coiled-coils or other helix-helix interfaces

Ligands and Environments

1 bound ligand:
Ligand CA 6 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL41XU
Spacegroup: P21
Unit cell:
a: 48.299Å b: 169.506Å c: 68.538Å
α: 90° β: 102.38° γ: 90°
R-values:
R R work R free
0.251 0.251 0.308
Expression system: Escherichia coli

Quick links

Citations

89 review citations

Troponin: the biomarker of choice for the detection of cardiac injury.
Babuin et al. (2005)
88 more

82 mentions without citation

Thin filament mutations: developing an integrative approach to a complex disorder.
Tardiff JC. (2011)
81 more