› 1j4x

X-ray diffraction
2.75Å resolution

HUMAN VH1-RELATED DUAL-SPECIFICITY PHOSPHATASE C124S MUTANT-PEPTIDE COMPLEX

Released:
DOI: 10.2210/pdb1j4x/pdb
PDB entry 1j4x coloured by chain, front view.
PDB entry 1j4x coloured by chain, side view.
PDB entry 1j4x coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis for the recognition of a bisphosphorylated MAP kinase peptide by human VHR protein Phosphatase.
Schumacher MA, Todd JL, Rice AE, Tanner KG, Denu JM
Biochemistry 41 3009-17 (2002)
PMID: 11863439

Function and Biology Details

Reactions catalysed: 
[a protein]-serine/threonine phosphate + H(2)O = [a protein]-serine/threonine + phosphate
Protein tyrosine phosphate + H(2)O = protein tyrosine + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156341 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Dual specificity protein phosphatase 3 Chain: A
Molecule details ›
Chain: A
Length: 184 amino acids
Theoretical weight: 20.35 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P51452 (Residues: 2-185; Coverage: 100%)
Gene names: DUSP3, VHR
Sequence domains: Dual specificity phosphatase, catalytic domain
Structure domains: Protein tyrosine phosphatase superfamily
DDE(AHP)(TPO)G(PTR)VATR Chain: D
Molecule details ›
Chain: D
Length: 11 amino acids
Theoretical weight: 1.41 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

No bound ligands
3 modified residues:
Ligand AHP 1 x AHP
Ligand TPO 1 x TPO
Ligand PTR 1 x PTR

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL7-1
Spacegroup: P212121
Unit cell:
a: 34.39Å b: 55.77Å c: 101.13Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.192 0.188 0.26
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

7 review citations

Protein tyrosine phosphatases: from genes, to function, to disease.
Tonks NK. (2006)
6 more

13 mentions without citation

Structural and functional analysis of PTPMT1, a phosphatase required for cardiolipin synthesis.
Xiao et al. (2011)
12 more