› 1jai

X-ray diffraction
1.8Å resolution

H-RAS P21 PROTEIN MUTANT G12P, COMPLEXED WITH GUANOSINE-5'-[BETA,GAMMA-METHYLENE] TRIPHOSPHATE AND MANGANESE

Released:
DOI: 10.2210/pdb1jai/pdb
PDB entry 1jai coloured by chain, front view.
PDB entry 1jai coloured by chain, side view.
PDB entry 1jai coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The role of the metal ion in the p21ras catalysed GTP-hydrolysis: Mn2+ versus Mg2+.
Schweins T, Scheffzek K, Assheuer R, Wittinghofer A
J Mol Biol 266 847-56 (1997)
PMID: 9102473

Function and Biology Details

Reaction catalysed: 
GTP + H(2)O = GDP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-133977 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
GTPase HRas, N-terminally processed Chain: A
Molecule details ›
Chain: A
Length: 166 amino acids
Theoretical weight: 18.92 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P01112 (Residues: 1-166; Coverage: 88%)
Gene names: HRAS, HRAS1
Sequence domains: Ras family
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

2 bound ligands:
Ligand MN 1 x MN
Ligand GCP 1 x GCP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ELLIOTT
Spacegroup: P3221
Unit cell:
a: 40Å b: 40Å c: 160.4Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.2 0.2 0.29
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Nucleoside 5'-triphosphates: self-association, acid-base, and metal ion-binding properties in solution.
Sigel et al. (2005)
3 more

2 mentions without citation

Predicting new indications for approved drugs using a proteochemometric method.
Dakshanamurthy et al. (2012)
1 more