› 1jmc

X-ray diffraction
2.4Å resolution

SINGLE STRANDED DNA-BINDING DOMAIN OF HUMAN REPLICATION PROTEIN A BOUND TO SINGLE STRANDED DNA, RPA70 SUBUNIT, RESIDUES 183-420

Released:
DOI: 10.2210/pdb1jmc/pdb
PDB entry 1jmc coloured by chain, front view.
PDB entry 1jmc coloured by chain, side view.
PDB entry 1jmc coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure of the single-stranded-DNA-binding domain of replication protein A bound to DNA.
Bochkarev A, Pfuetzner RA, Edwards AM, Frappier L
Nature 385 176-81 (1997)
PMID: 8990123

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-114018 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
Replication protein A 70 kDa DNA-binding subunit, N-terminally processed Chain: A
Molecule details ›
Chain: A
Length: 246 amino acids
Theoretical weight: 27.52 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P27694 (Residues: 181-422; Coverage: 39%)
Gene names: REPA1, RPA1, RPA70
Sequence domains:
Structure domains: Nucleic acid-binding proteins
DNA (5'-D(*CP*CP*CP*CP*CP*CP*CP*C)-3') Chain: B
Molecule details ›
Chain: B
Length: 8 nucleotides
Theoretical weight: 2.27 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P212121
Unit cell:
a: 34.25Å b: 77.99Å c: 95.36Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.212 0.1 0.33
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

44 review citations

Hfq and its constellation of RNA.
Vogel et al. (2011)
43 more

59 mentions without citation

Nucleic acid recognition by OB-fold proteins.
Theobald et al. (2003)
58 more