› 1jtv

X-ray diffraction
1.54Å resolution

Crystal structure of 17beta-Hydroxysteroid Dehydrogenase Type 1 complexed with Testosterone

Released:
DOI: 10.2210/pdb1jtv/pdb
PDB entry 1jtv coloured by chain, front view.
PDB entry 1jtv coloured by chain, side view.
PDB entry 1jtv coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Pseudo-symmetry of C19 steroids, alternative binding orientations, and multispecificity in human estrogenic 17beta-hydroxysteroid dehydrogenase.
Gangloff A, Shi R, Nahoum V, Lin SX
FASEB J 17 274-6 (2003)
PMID: 12490543

Function and Biology Details

Reactions catalysed: 
Testosterone + NAD(P)(+) = androst-4-ene-3,17-dione + NAD(P)H
17-beta-estradiol + NAD(P)(+) = estrone + NAD(P)H
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146840 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
17-beta-hydroxysteroid dehydrogenase type 1 Chain: A
Molecule details ›
Chain: A
Length: 327 amino acids
Theoretical weight: 34.89 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P14061 (Residues: 2-328; Coverage: 100%)
Gene names: E17KSR, EDH17B1, EDH17B2, EDHB17, HSD17B1, SDR28C1
Sequence domains: short chain dehydrogenase
Structure domains: NAD(P)-binding Rossmann-like Domain

Ligands and Environments

2 bound ligands:
Ligand TES 1 x TES
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X8C
Spacegroup: C2
Unit cell:
a: 122.369Å b: 43.944Å c: 60.614Å
α: 90° β: 99.24° γ: 90°
R-values:
R R work R free
0.196 0.196 0.208

Quick links

Citations

14 review citations

Gonadal steroid hormones and the hypothalamo-pituitary-adrenal axis.
Handa et al. (2014)
13 more

13 mentions without citation

The structural biology of oestrogen metabolism.
Thomas et al. (2013)
12 more