› 1k2a

X-ray diffraction
1Å resolution

Modified Form of Eosinophil-derived Neurotoxin

Released:
DOI: 10.2210/pdb1k2a/pdb
PDB entry 1k2a coloured by chain, front view.
PDB entry 1k2a coloured by chain, side view.
PDB entry 1k2a coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystallographic and functional studies of a modified form of eosinophil-derived neurotoxin (EDN) with novel biological activities.
Chang C, Newton DL, Rybak SM, Wlodawer A
J Mol Biol 317 119-30 (2002)
PMID: 11916383

Function and Biology Details

Reaction catalysed: 
(1a) an (RNA) containing cytidine = an (RNA)-3'-cytidine-2',3'-cyclophosphate + a 5'-hydroxy-ribonucleotide-3'-(RNA)
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-145194 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Non-secretory ribonuclease Chain: A
Molecule details ›
Chain: A
Length: 136 amino acids
Theoretical weight: 15.72 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P10153 (Residues: 26-161; Coverage: 100%)
Gene names: EDN, RNASE2, RNS2
Sequence domains: Pancreatic ribonuclease
Structure domains: Ribonuclease A-like domain

Ligands and Environments

1 bound ligand:
Ligand SO4 2 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X9B
Spacegroup: P212121
Unit cell:
a: 42.138Å b: 52.558Å c: 56.627Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.135 0.132 0.169
Expression system: Escherichia coli

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Citations

2 review citations

Cytotoxic ribonucleases: molecular weapons and their targets.
Makarov et al. (2003)
1 more

4 mentions without citation

Coarse-grained description of protein internal dynamics: an optimal strategy for decomposing proteins in rigid subunits.
Potestio et al. (2009)
3 more