› 1k39

X-ray diffraction
3.29Å resolution

The structure of yeast delta3-delta2-enoyl-COA isomerase complexed with octanoyl-COA

Released:
DOI: 10.2210/pdb1k39/pdb
PDB entry 1k39 coloured by chain, front view.
PDB entry 1k39 coloured by chain, side view.
PDB entry 1k39 coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
The crystal structure of delta3-delta2-enoyl-CoA isomerase
Mursula AM, van Aalten DMF, Hiltunen JK, Wierenga RK
J. Mol. Biol. 309 845-853 (2001)

Function and Biology Details

Reaction catalysed: 
A (3E)-alk-3-enoyl-CoA = a (2E)-alk-2-enoyl-CoA
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-170260 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
3,2-trans-enoyl-CoA isomerase Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 280 amino acids
Theoretical weight: 31.72 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: Q05871 (Residues: 1-280; Coverage: 100%)
Gene names: ECI1, YLR284C
Sequence domains: Enoyl-CoA hydratase/isomerase
Structure domains: 2-enoyl-CoA Hydratase; Chain A, domain 1

Ligands and Environments

2 bound ligands:
Ligand PO4 3 x PO4
Ligand CO8 3 x CO8
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: MAX II BEAMLINE I711
Spacegroup: P41212
Unit cell:
a: 116.65Å b: 116.65Å c: 216.78Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.208 0.205 0.262
Expression system: Escherichia coli

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