› 1k3y

X-ray diffraction
1.3Å resolution

Crystal Structure Analysis of human Glutathione S-transferase with S-hexyl glutatione and glycerol at 1.3 Angstrom

Released:
DOI: 10.2210/pdb1k3y/pdb
PDB entry 1k3y coloured by chain, front view.
PDB entry 1k3y coloured by chain, side view.
PDB entry 1k3y coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
1.3-A resolution structure of human glutathione S-transferase with S-hexyl glutathione bound reveals possible extended ligandin binding site.
Le Trong I, Stenkamp RE, Ibarra C, Atkins WM, Adman ET
Proteins 48 618-27 (2002)
PMID: 12211029

Function and Biology Details

Reaction catalysed: 
RX + glutathione = HX + R-S-glutathione
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140083 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glutathione S-transferase A1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 221 amino acids
Theoretical weight: 25.54 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P08263 (Residues: 2-222; Coverage: 100%)
Gene name: GSTA1
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand GTX 2 x GTX
1 bound ligand:
Ligand GOL 3 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-2
Spacegroup: C2
Unit cell:
a: 99.38Å b: 93.77Å c: 51.627Å
α: 90° β: 93.39° γ: 90°
R-values:
R R work R free
0.137 0.136 0.205
Expression system: Escherichia coli

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Citations

1 review citation

Human cytosolic glutathione-S-transferases: quantitative analysis of expression, comparative analysis of structures and inhibition strategies of isozymes involved in drug resistance.
Mohana et al. (2017)
23 other articles

20 mentions without citation

Interactions of glutathione transferases with 4-hydroxynonenal.
Balogh et al. (2011)
19 more